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. 2013 Jul 7;288(32):22915–22929. doi: 10.1074/jbc.M113.484337

FIGURE 3.

FIGURE 3.

Effect of class IIa inhibition on muscle differentiation and epigenetic modifications. A–D, histological analysis of control (A) and ischemic muscle (3, 14, and 21 days, B–D, respectively) treated with vehicle or the class IIa HDAC-specific inhibitor MC1568 (H&E-stained sections). The graph (A, right panel) shows that the increase in immature centronucleated fibers is higher in MC1568-treated ischemic muscles than in vehicle-treated ischemic muscles. *, p < 0.05 versus control. E, Western blot showing MHC levels during post-ischemic regeneration in adductor muscles from untreated and MC1568-treated mice. The graph shows the relative level of MHC normalized to loading control (GAPDH). *, p < 0.05 versus control. F, Western blot of histone modifications showing an increase, soon after ischemia, in the level of H3K9ac, H3S10p, and H3K4m3 whereas H3K20m3 level was decreased. G, Western blot of histone modifications (H3K9ac, H3S10p) showing the effect of MC1568 treatment in ischemic samples. Band density analyses are shown in the bottom panels. *, p < 0.05 versus control; #, versus ischemia 3 days; °, versus ischemia 14 days. Error bars, S.E.