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. 2013 Jul 7;288(32):22915–22929. doi: 10.1074/jbc.M113.484337

FIGURE 9.

FIGURE 9.

Evaluation of the effect of eNOS (adenovirus eNOS-S1177D) overexpression in ischemic mice treated or not with MC1568 and MS275 at 14 days. Adenovirus GFP-CMVnull-injected mice were used as controls. A, representative images of H&E-stained sections. B, analysis of centronucleated fibers. C, evaluation of degenerative areas. *, p < 0.05 versus control; §, p < 0.05 versus MC; °, p < 0,05 versus MS. D, evaluation of centronucleated fibers at 14 days of ischemia in vehicle and l-NAME-treated mice. E, measurement of limb perfusion by LISCA immediately after ischemia and at 14 days after ischemia in AdGFP-CMV-eNOS- or AdGFP-CMVnull-infected adductors treated or not with MC1568 or MS275. *, p < 0.05 versus control; §, p < 0.05 versus MC. Representative images are also provided of animals soon after ischemia and at 14 days. F, representative images of GFP-positive fibers in ischemic adductor muscles infected with AdGFP-CMV-eNOS or AdGFP-CMVnull. The graph shows the percentage of GFP-positive fibers in ischemic untreated or MC1568- or MS275-treated mice. Error bars, S.E.