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. 2013 Jun 25;288(32):22930–22941. doi: 10.1074/jbc.M112.404186

FIGURE 1.

FIGURE 1.

The presence of bikunin (A) and 4-sulfated CS linkage regions (B) in proteoglycan-enriched fractions of urine and plasma after digestion with chondroitinase ABC as determined by ELISA is shown. C, the presence of 4-sulfated CS linkage regions attached to bikunin in proteoglycan-enriched urine as determined by sandwich ELISA using a bikunin antibody (ab43073) capture and detected with the 4-sulfated CS linkage region antibody (2B6). Data are presented as mean ± S.D. (error bars) (n = 4). Western blot analyses of bikunin and 4-sulfated CS linkage regions in proteoglycan-enriched urine (D) and analyses of bikunin, 4-sulfated CS linkage regions, HC2, and HC1 in proteoglycan-enriched plasma (E) are shown. Samples were analyzed without and with chondroitinase ABC digestion (C'ase ABC) or NaOH treatment.