FIGURE 9.
Structure-based sequence alignment of AlyA1PL7 and AlyA5. The sequences are aligned with those from two other PL7 alginate lyases with known structure, AlyPG from Corynebacterium sp. ALY-1 (PDB code 1UAI) and A1-II′ from Sphingomonas sp. A1 (PDB code 2ZAA). The secondary structures of AlyA1PL7 and AlyA5 are shown above and below the alignment, respectively. Conserved amino acids in white letters highlighted on the red background are identical and those in red letters are similar. α-Helices are represented as schematically, and β-turns are marked with TT. Green triangles indicate the conserved residues involved in the catalytic machinery. This figure has been generated using the program ESPRIPT (72).