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(A) Saturation binding of 125I-rhβ2GPI to DS and moAb1 to β2GPI. Saturation binding of 125I-rhβ2GPI (1.56-200 nM) to immobilized moAb1 (▴), DS (▾), and BSA (▪) was performed as indicated in Methods. (B) Direct Binding of 125I-rhβ2GPI to microtiter wells coated with PS, DS, or BSA. The amount of 125I-rhβ2GPI bound was determined as described in Methods.
