TABLE 2.

Kinetic parameters for NAD of Ugd_K-12 and Ugd_K30 isolated from CWG876

	kcat	Km	Ksi
	s−1	mm	mm
UgdK-12	7.1 ± 0.5	0.62 ± 0.07	1.4 ± 0.2
UgdK30	4.2 ± 0.2	0.31 ± 0.03	8.7 ± 1.6
UgdK-12 Y71Fa	7.3 ± 0.9	0.50 ± 0.10	1.4 ± 0.3
UgdK-12 K323A	2.7 ± 0.2	0.43 ± 0.05	2.8 ± 0.4
UgdK-12 + [ATP] (μm)
25	5.9 ± 0.4	0.45 ± 0.05	2.6 ± 0.3
50	6.2 ± 0.4	0.62 ± 0.06	2.8 ± 0.4
100	5.3 ± 0.2	0.45 ± 0.03	5.4 ± 0.7
250	5.1 ± 0.3	0.45 ± 0.04	13.4 ± 3.7
500	5.0 ± 0.2	0.35 ± 0.03	24.9 ± 7.9
750	5.6 ± 0.3	0.44 ± 0.04	30.9 ± 15.2
1000	5.5 ± 0.2	0.38 ± 0.03	47.4 ± 27.0

^a Ugd_K-12 Y71F was tested in duplicate; all others were tested in triplicate.