FIGURE 6.

Electrophysiological characterization of Ugr 9-1 action on human ASIC3 expressed in the X. laevis oocyte. Acid-induced currents were evoked by pH drop; the time intervals between the recordings were about 1 to 2 min, and the holding potential was −50 mV. A, effect on both current components by application of Ugr 9-1 at a concentration of 40 μm; B, effect on the transient current by application of Ugr 9-1 at a concentration of 20 μm; C, effect on the sustained current by application of Ugr 9-1 at a concentration of 10 μm; D and E, dose-response curves for the Ugr 9-1 inhibitory activity separately calculated for the transient (D) and sustained (E) components. Each point is the mean ± S.D. of 3–7 measurements. Data were fitted by the logistic equation.