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. 2013 Jun 20;288(32):23128–23140. doi: 10.1074/jbc.M113.491654

FIGURE 1.

FIGURE 1.

Regulation of Kv2.2 by pyruvate-isocitrate cycling and regulation of GSIS by Kv2.2. 832/13 cells were treated with siControl (siCont), siCIC, or siICDc for 72 h, and total RNA was isolated for qRT-PCR analyses of CIC, ICDc, Kv2.1, and Kv2.2 expression levels. Expression levels for siICDc- (A) and siCIC-treated (B) 832/13 cells are shown. Data represent four and three independent experiments, respectively, each performed in duplicate. *, p < 0.001; #, p < 0.01. 832/13 cells were either left untreated or transduced with siKV2.1, siKv2.2, or a control (siControl) duplex and cultured for 3 days before qRT-PCR (C) or GSIS (D) analyses. Data represent three independent experiments, each performed in triplicate. *, p < 0.001; #, p < 0.01. Error bars represent S.E. uU, microunits.