FIGURE 5.

Kv2.2 overexpression rescues the inhibitory effects of siICDc on GSIS in primary islets. Freshly isolated rat islets were transduced with an equal dose of Ad-siControl (siCont) or Ad-siICDc (siICDc) and subsequently incubated for a total of 72 h. A, for the last 48 h of culture, islets were treated with RIP-GFP. After trypsinization and FACS sorting, GFP-positive β-cells were analyzed for Kv2.2 expression. For B, C, and D, the transduced islets were exposed to equal doses of AdCMV-βgal (β-gal) or AdCMV-hKv2.2 (kv2.2) for the last 40 h of culture. qRT-PCR analyses of Kv2.2 (B) and ICDc (C) mRNA levels are shown. D, glucose-stimulated insulin secretion. Data represent average ± S.E. (error bars) of three independent experiments performed in triplicate. *, p < 0.001; #, p < 0.01; @, p < 0.05. uU, microunits; ns, not significant.