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. 2013 Jun 28;288(32):23161–23170. doi: 10.1074/jbc.M113.487157

FIGURE 2.

FIGURE 2.

Effect of RSV on MAT2BV1 and V2 promoter activity and MAT2B mRNA stability. A, HepG2 cells were transfected with V1 or V2 promoter constructs and SIRT1 siRNA (SIRT1si) or scramble siRNA (SC) prior to treatment with RSV for 12 h as described under “Experimental Procedures.” SIRT1 knockdown lowered basal V1 and V2 promoter activity and blunted the RSV-mediated increase in V1 and V2 promoter activity. B, results of mRNA stability assays in HepG2 cells performed as described under “Experimental Procedures” are shown. DMSO, dimethyl sulfoxide. C, RNP-IP analysis was performed as described under “Experimental Procedures” to demonstrate HuR binding to the MAT2B 3′-UTR sequence in HepG2 cells. Results are mean ± S.E. (error bars) from three independent experiments. *, p < 0.05 versus respective controls; †, p < 0.05 versus either V1+SIRT1 siRNA or V2+SIRT1 siRNA; ‡, p < 0.05 versus either V1+RSV+SC or V2+RSV+SC treatment.