FIGURE 6.

PDCL3 potentiates VEGF-mediated activation and signaling of VEGFR-2. PAE cells coexpressing VEGFR-2 with an empty vector or with PDCL3 were serum-starved and stimulated or left unstimulated with VEGF for the indicated times. Whole cell lysates were subjected Western blot analysis using anti-phospho-Tyr-1212-VEGFR-2 antibody (A), anti-phospho-Tyr-1054-VEGFR-2 antibody (B), anti-phospho-Tyr-1173-VEGFR-2 antibody (C), anti-VEGFR-2 (D), anti-phospho-Tyr-783-PLCγ1 antibody (E), PLCγ1 antibody (F), and anti-PDCL3 antibody (G). H, representative of three independent experiments corresponding to phosphorylation of VEGFR-2 at the multiple sites and phosphorylation of PLCγ1.