FIGURE 2.

Analysis of TXNIP promoter region conferring FOXO1 effects. A, alignment of the proximal human, rat, and mouse TXNIP promoter sequence reveals a highly conserved ChoRE with an E-box repeat (black) and putative FOXO binding site (white). B, INS-1 cells were co-transfected with FOXO1 expression plasmid or LacZ control plasmid and luciferase reporter constructs driven by the full-length TXNIP promoter or a number of TXNIP promoter deletions (D1-mutD4). C, INS-1 cells were co-transfected with FOXO1 expression plasmid or LacZ control plasmid and reporter constructs containing luciferase driven by the heterologous SV40 promoter with or without upstream ChoRE repeat (SV40-ChoRE). Luciferase activities are expressed as percentage of the LacZ control; bars represent means ± S.E. (error bars); n = 3–4.