FIGURE 5.

Thr-55 phosphorylation is important for SHP repression activity. A–F, HepG2 cells were transfected with plasmids as indicated and treated with either FGF19 (8 h) or CDCA overnight and reporter assays were performed. The triangle indicates increasing amounts of the SHP expression plasmids. The values for firefly luciferase activities were normalized by β-galactosidase activities. The mean ± S.E. are plotted (n = 3). G, expression levels of SHP WT and the mutants from cell extracts are shown.