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Thr-55 phosphorylation increases SHP interaction with repressive chromatin modifiers and their recruitment to BA-responsive target genes. A, co-IP: transcriptional regulators were immunoprecipitated and the presence of FLAG-SHP was detected by M2 antibody. B–F, sequential liver chromatin IP. B, experimental outline. C–F, chromatin was immunoprecipitated with M2 antibody, eluted, and re-precipitated with antibody as indicated. Precipitated genomic DNA was quantified by semi-qPCR.
