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. 2013 Jun 26;288(32):23368–23379. doi: 10.1074/jbc.M113.460394

FIGURE 1.

FIGURE 1.

Ang II activates Wnt/β-catenin signaling in cultured podocytes. A, real-time PCR analysis showing the induction of Wnt1 and Wnt3 mRNA at different time points after Ang II treatment as indicated, the asterisk indicates p < 0.05 compared with controls (n = 3). B, Western blot showing Wnt1 protein abundance in podocytes after Ang II treatment, β-actin was probed as normalization. C and D, Western blot showing p-β-catenin (Ser-33/37) and p-β-catenin (Ser-675) abundance after Ang II treatment, β-actin was probed as normalization (C); semi-quantitative analysis for p-β-catenin (Ser-33/37) and p-β-catenin (Ser-675) abundance (D). E, Western blot showing β-catenin abundance in podocyte cytoplasm or nuclei after Ang II treatment. F, representative immunofluorescent staining images showing β-catenin underwent nuclear translocation after Ang II treatment. Arrows indicate β-catenin positive nuclei. Cells were counterstained with DAPI for nuclear visualization. G, the graph showing the result for β-catenin-mediated gene transcriptional activity assay. Podocytes were transfected with TOP-flash reporter or FOP-flash plasmid. Relative luciferase activity was reported as the mean ± S.E., the asterisk indicates p < 0.05 versus control (n = 3). H, Western blotting assay showing the abundance of the phosphorylated LRP6 and quantitative analysis for p-LRP6 in mouse podocytes after Ang II treatment.