FIGURE 5.

Binding of G-actin to immobilized C28 peptide. A, representative sensorgram of G-actin binding to immobilized C28 peptide at an immobilization level of ∼650 RU is shown. G-actin was assayed in the range 0.5–16 μm in a modified Buffer G (2 mm Tris-HCl, 70 μm Ca²⁺, pH 7.7 at 25 °C). The time for the association phase was set to 60 s. Curves were corrected for bulk effects by simple subtraction of the corresponding control sensorgrams. B, nonspecific binding of G-actin to the C28 peptide.