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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Nat Struct Mol Biol. 2012 Oct 14;19(11):1168–1175. doi: 10.1038/nsmb.2400

Figure 3.

Figure 3

riRNAs recognize complementary sequences in the 3'UTRs of target mRNAs. (a) Schematic structure of selected RA negatively-regulated transcripts, Tdgf1 and Nanog. The wave line denotes the 3’UTR. (b) RNA immunoprecipitation showed RA-dependent and DR2 Alu-dependent interaction of AGO3 with the 3’UTRs of Nanog and Tdgf1 mRNAs. (c) RT-qPCR showed that RA treatment decreased the transcript levels of examined genes and the specific siRNA against AGO3 reversed the effect. Over-expression of full-length AluSx transcripts (d) or synthesized 56 nt. riRNA (e) in Ntera2 cells mimicked the RA action in decreasing transcript levels of those genes. (f) Two siRNAs against DR2 Alu transcripts abolished RA-mediated downregulation of those transcripts. (mean±SEM; *p<0.05, **p<0.01)