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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Nat Struct Mol Biol. 2012 Oct 14;19(11):1168–1175. doi: 10.1038/nsmb.2400

Figure 4.

Figure 4

Role of riRNA complementary sequence in post-transcriptional regulation. (a) Luciferase assays using constructs harboring 3’UTRs of Nanog, Tdgf1 or Ventx showed RA-dependent reduction in luciferase activity in Ntera2 cells. Expression of either full-length AluSx transcripts or synthesized short riRNAs gave similar results. (b) Luciferase assays showed that when the putative ~50bp riRNA targeting sequence was deleted from the Ventx or Nanog 3'UTR luciferase reporters, they no longer responded to RA treatment or over-expression of full-length AluSx or riRNAs. (c) In luciferase assays using the wild-type Nanog 3’UTR luciferase reporter, knockdown of AGO3 and DR2 Alu restored the luciferase activity in the presence of RA. (mean±SEM; *p<0.05, **p<0.01)