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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Nat Struct Mol Biol. 2012 Oct 14;19(11):1168–1175. doi: 10.1038/nsmb.2400

Figure 5.

Figure 5

Role of the AGO3-associated decapping complex in post-transcriptional regulation. (a) Silver-staining of anti-AGO3 immunoprecipitates in Ntera2 cells. The same precipitates were subsequently analyzed by mass spectrometry. (b) Co-immunoprecipitation with the AGO3 antibody (4B1-F6) confirmed the interaction between AGO3 and EDC-4 (right upper panel). 4B1-F6 proved to be applicable in the immunoprecipitation assays while another AGO3 polyclonal IgG (p-anti-Ago3) manufactured for immunoblotting purposes effectively served only as a negative control (right lower panel). (c) RNA immunoprecipitation revealed RA-dependent and Alu-dependent recruitment of DCP1a and DCP2 proteins to the target mRNAs. (d) RT-qPCR showed that Ntera2siRNAs knock-down of DCP1a, DCP2, EDC4 and XRN1 restored the transcript levels of indicted genes in Ntera2 cells. (mean±SEM; *p<0.05, **p<0.01)