Skip to main content
. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Biochem J. 2011 May 15;436(1):91–100. doi: 10.1042/BJ20101891

Figure 3. Direct modulation of hepatic insulin signalling by macrophages from PON2-def mice is associated with altered inflammatory response.

Figure 3

Peritoneal macrophages obtained from PON2-def (A) and PON2-def/apoE−/− (B) mice maintained on a standard chow diet were activated (0.1 µg/ml LPS) and allowed to recover for 18 h. FL83B hepatocytes were then treated with CM from macrophages for 18 h and impaired insulin signalling was evaluated by detection of phosphorylation of IRS-1 at Ser307. (C) LPS-elicited production of ROS was measured at the indicated times by quantifying fluorescence emitted by ROS-mediated oxidation of DCFH-DA (2′,7′-dichlorodihydrofluorescein diacetate) in macrophages. ○, control; ●, PON2-def. (D) mRNA expression levels of the indicated genes determined using quantitative RT (reverse transcription)–PCR. Values are means ± S.E.M. *P < 0.05.