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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Biochem J. 2011 May 15;436(1):91–100. doi: 10.1042/BJ20101891

Figure 5. Macrophage-mediated NO generation and ONOO−formation.

Figure 5

Peritoneal macrophages from PON2-def mice and littermate control were treated overnight with LPS (0.1 µg/ml) for determination of supernatant nitrite concentrations (A) and cellular nitrotyrosine content (B). Peritoneal macrophages from PON2-def/apoE−/− mice and littermate controls were treated overnight with LPS (0.1 µg/ml) for determination of supernatant nitrite concentrations (C) and cellular nitrotyrosine content (D). Protein nitrotyrosine levels were determined in vivo using serum from the indicated standard-diet-fed mice (E). Peritoneal macrophages obtained from PON2-def and littermate control mice were treated overnight with uric acid (10µM) and TNFα expression levels were determined following a subsequent 2 h LPS (0.1 µg/ml) treatment (F). Values are means ± S.D. *P < 0.05. AU, arbitrary units.