. Author manuscript; available in PMC: 2014 Aug 7.

Published in final edited form as: Tetrahedron Lett. 2013 Jun 10;54(32):4300–4302. doi: 10.1016/j.tetlet.2013.06.008

Table 1.

Cytotoxicity of compounds 1 and 2 against two human tumor cell lines^a

Compound	IC₅₀ values in µM

	MDA-MB-435^b	SW-620^c
Waol A (1)	39.6	13.8
trans-Dihydrowaol A (2)	>40	>40

Positive controls were vinblastine and bortezomib. Vinblastine was tested at concentrations of 3 ng/mL and 1 ng/mL: MDA-MB-435 cells had 37% and 99% viable cells; SW620 cells had 76% and 90% viable cells; respectively. Bortezomib was tested at concentrations of 5 nM and 2.5 nM: MDA-MB-435 cells had 90% and 91% viable cells; SW620 cells had 79% and 71% viable cells; respectively.

melanoma

colon tumor cell lines were tested using published protocols.^3,20