TABLE 4.
The number of metabolites that were different before and after the PAPOLG genotypes 1 and 2 intervention in volunteers within the high-glucoraphanin broccoli arm (n = 19)1
| Genotype 1 |
Genotype 2 |
|||
| Pathway2 | Metabolites decreased by intervention | Metabolites increased by intervention | Metabolites decreased by intervention | Metabolites increased by intervention |
| Amino acids (85) | 3 | 1 | 7 | 10 |
| Carbohydrates (23) | 0 | 0 | 0 | 0 |
| Cofactors and vitamins (15) | 1 | 0 | 0 | 2 |
| Energy (9) | 0 | 0 | 0 | 1 |
| Acylcarnitines (12) | 3 | 0 | 2 | 0 |
| Lipids (129) | 54 | 0 | 1 | 51 |
| Nucleotides (14) | 4 | 0 | 0 | 3 |
| Peptides (14) | 0 | 0 | 0 | 1 |
| Xenobiotics (46) | 0 | 0 | 2 | 2 |
| Total (347) | 66 | 82 | ||
| Median number after Y scrambling | 143 | 114 | ||
| 5 | <0.002 | <0.004 | ||
All values are the number of metabolites within each class that reached the achieved statistical threshold (P < 0.05). Details of the metabolites and P values are provided elsewhere (see Supplemental Table S5 under “Supplemental data” in the online issue). t Tests were used to analyze differences in the log2 ion intensity of each metabolite within each genotype before and after the intervention.
Numbers in parentheses are the total number of metabolites within each class.
The value was derived after 2048 permutations of the data set.
The value was derived after 256 permutations of the data set.
The probability that the total number of metabolites found to be different would have occurred by chance, estimated from the frequency distribution of metabolites that reached the statistical threshold (P < 0.05) after permutations of the data set.