Table 3.
Quantitative RT-PCR determination of the principal cytochrome P450s (Cyp) induced by standard inducers and flavone-8-acetic acid (FAA)a
| Inducer | 1a1b | 1a2 | 1b1 | 2e1 | 2b10 | 2d9 | 3a11 |
|---|---|---|---|---|---|---|---|
| Phenobarbital | 0.07 c± 0.04 | 0.75 ± 0.1 | 0.2 ± 0.09 | 1 ± 0.2 | 3.2 ± 0.7 | 2.5 ± 0.4 | 1.6 ± 0.1 |
| β-naphthoflavone | 450 ± 330 | 30 ± 9 | 28 ± 8 | 1.1 ± 0.05 | 1.6 ± 0.2 | 2.4 ± 0.5 | 1.2 ± 0.3 |
| 3-methylcholanthrene | 11000 ±2900 | 180 ± 17 | 680 ± 120 | 1.5 ± 0.1 | 2.4 ± 0.7 | 2.9 ± 0.4 | 1.5 ± 0.4 |
| Dexamethasone | 24 ± 16 | 4 ± 2 | 3.1 ± 1.7 | 0.78 ± 0.02 | 1.7 ± 0.2 | 18 ± 3.9 | 3.6 ± 1.0 |
| Aroclor 1254 | 7800 ± 2900 | 200 ± 39 | 370 ± 89 | 0.4 ± 0.1 | 8.4 ± 1.4 | 6.0 ± 1.4 | 5.5 ± 1.6 |
| FAA 1 treatment | 0.003 ±0.001 | 0.08 ± 0.01 | 0.1 ± 0.01 | 0.6 ± 0.1 | 0.5 ± 0.08 | 0.5 ± 0.1 | 0.7 ± 0.1 |
| FAA 3 treatments | 0.6 ± 0.3 | 1.9 ± 1.1 | 1.1 ± 0.3 | 2.1 ± 0.5 | 3.2 ± 1.5 | 2.3 ± 0.7 | 2.2 ± 0.4 |
a
Levels of Cyp transcripts were determined by quantitative RT-PCR as described in the Materials and Methods section.
b
The mouse Cyp orthologs correspondance with the human CYPs are as follows (mouse Cyp, human CYP): Cyp1a2, CYP1A2; Cyp1b1, CYP1B1; Cyp2b9, CYP2B6; Cyp2c39, CYP2C19; Cyp2d9, CYP2D6; Cyp2e1, CYP2E1; Cyp3a11, CYP3A4.
c
Fold-induction relative to their respective controls (saline or corn oil). Mean ± SEM of 5 independent determinations.