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. 2013 Aug 14;8(8):e71359. doi: 10.1371/journal.pone.0071359

Figure 7. Effect of stretch-induced eNOS activation and NO production on WPB exocytosis and leukocyte adhesion.

Figure 7

(A) Quantitative analysis of stretch-induced vWF release from HUVECs pretreated with SNAP (50 µM), L-NAME (1 mM) or vehicle (DMSO). (B) Left: quantitative analysis of stretch-induced vWF release from HUVECs expressing scrambled (Scr) or eNOS-targeting (eNOS KD) shRNAs. Right: Western blots of the knockdown efficiency of shRNAs targeting eNOS in HUVECs. (C) Left: HL-60 cell adhesion to HUVEC monolayers after stretch, pretreated with SNAP (50 µM), L-NANE (1 mM) or vehicle (DMSO). Right: quantitative analysis of HL-60 adhesion. (D) Left: stretch-induced HL-60 cell adhesion to HUVECs expressing scrambled (Scr) or eNOS-targeting (eNOS KD) shRNAs. Right: quantitative analysis of HL-60 adhesion. Results are representative of 3 individual experiments and expressed as mean ± S.D. (n = 4). **P<0.01.