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. 2013 Aug 14;8(8):e71764. doi: 10.1371/journal.pone.0071764

Figure 2. Effect of C9orf86 knockdown on cell proliferation in human breast cancer cells.

Figure 2

(A) Forty-eight hours post-transfection, expression of C9orf86 in MCF-7 and SK-BR-3 cells was quantified by western blot analysis. GAPDH was used as a loading control. (B) Colony formation assay. Twenty-four hours post-transfection, MCF-7 and SK-BR-3 cells were seeded into 6-well plates with complete medium and incubated at 37°C for 2 weeks. (C) MTT assay. (D) WST-1 assay. Twenty-four hours post-transfection, MCF-7 and SK-BR-3 cells were seeded into 96-well plates. The colony formation assay (B), MTT assay (C) and WST-1 assay (D) showed that knockdown of C9orf86 in MCF-7 and SK-BR-3 cells resulted in inhibition of cell growth in vitro. All data are shown as mean ± SD of triplicate experiments. *P<0.05. NC, negative control.