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qRT-PCR performed on 5 differentially expressed genes (Prol1, Muc1, Vcsa1, Fcnb, Il1b) for flaps undergoing both arterial ischemia and venous congestion for 4 hours compared to control. n = 5 and performed in duplicate. Data are expressed as means ± SE and analyzed by unpaired t-test. *P<0.05, **P<0.01, ***P<0.001, cf. control flaps; § P<0.05, cf. arterial ischemia flaps.
