Table 2. Ingenuity Pathway Analysis results for differentially expressed genes in rat composite microvascular flaps undergoing 4 hours of arterial ischemia and venous congestion compared to control flaps.

	Score	P-value	Genes, n
Arterial Ischemia
Top Associated Network Functions
Infectious Disease, antigen presentation, inflammatory response	33
Cellular compromise, cellular function and maintenance, inflammatory response	30
Cellular function and maintenance, cell-to-cell signaling and interaction, inflammatory response	28
Cellular movement, hematological system development and function, cell-to-cell signaling and interaction	28
Cellular movement, hematological system development and function, immune cell trafficking	25
Top biological functions
Inflammatory response		1.02E-21–1.48×10−04	108
Inflammatory disease		8.11E-16–1.03×10−04	122
Immunological disease		9.57E-14–1.48×10−04	112
Organismal injury and abnormalities		2.37E-12–1.48×10−04	53
Respiratory disease		4.10E-12–1.48×10−04	49
Cellular development		5.84E-24–1.52×10−04	112
Cellular movement		9.35E-18–1.39×10−04	83
Cellular function and maintenance		3.62E-15–1.52×10−04	87
Cell death		5.26E-15–1.48×10−04	130
Cell-to-cell signaling and interaction		6.45E-15–1.48×10−04	98
Hematological system development		8.74E-20–1.61×10−04	120
Tissue morphology		8.74E-20–7.04×10−05	74
Immune cell trafficking		9.35E-18–1.61×10−04	79
Hematopoiesis		2.40E-17–1.52×10−04	78
Cell-mediated Immune Response		8.18E-14–1.52×10−04	54
Top Canonical Pathways
Colorectal cancer metastasis signaling		9.65×10−7
Type I diabetes mellitus signaling		1.35×10−6
Production of nitric oxide and reactive oxygen species in macrophages		1.85×10−6
Role of macrophages, fibroblasts and endothelial cells in rheumatoid arthritis		2.22×10−6
Role of pattern recognition receptors in recognition of bacteria and viruses		2.57×10−6
Venous congestion
Top associated network functions
Renal and urological disease, cell death, embryonic development	41
Nutritional disease, immunological disease, gastrointestinal disease	32
Post-translational modification, cell death, nervous system development and function	32
Cell signaling, cardiovascular system development and function, connective tissue disorders	31
Genetic disorder, immunological disease, cardiovascular disease	30
Top biological functions
Inflammatory response		1.07E-28–2.44×10−07	262
Cancer		4.81E-27–1.72×10−07	423
Inflammatory disease		4.58E-25–2.59×10−07	384
Immunological disease		7.44E-23–2.30×10−07	326
Hematological disease		2.23E-17–3.17×10−07	250
Cellular movement		1.94E-34–3.17×10−07	285
Cellular growth and proliferation		6.15E-34–2.49×10−07	396
Cellular development		7.63E-27–2.47×10−07	352
Cell death		3.31E-23–2.53×10−07	371
Cell-to-cell signaling and interaction		1.05E-22–2.01×10−07	268
Hematological system development and function		3.36E-33–2.30×10−07	291
Immune cell trafficking		3.36E-33–2.27×10−07	196
Tissue morphology		4.73E-24–2.30×10−07	186
Organismal survival		9.46E-23–2.05×10−08	201
Tissue development		1.05E-22–2.13×10−07	198
Top Canonical Pathways
Type I diabetes mellitus signaling		1.05×10−10
Production of nitric oxide and reactive oxygen species in macrophages		1.28×10−7
Hepatic fibrosis/hepatic stellate cell activation		1.90×10−7
IL-10 signaling		3.96×10−7
T helper cell differentiation		5.02×10−7