Figure 2. PrP^CWD detection in CWD-inoculated dams.

(A) IHC PrP^CWD is demonstrated (red deposits) in a tonsilar lymphoid biopsy collected at 4 mpi (A3) and in the obex of a terminal TSE diseased dam at 23 mpi (A1). Absence of red deposits is shown in negative control tonsil (A4) and obex (A2). Picture objective is 20X (scale bar = 200 µm). (B) Western blot detection of PrP^CWD in obex tissue of a CWD-inoculated dam at 23 mpi (B lane 5) following PK digestion. Complete PK digestion of PrP^C is shown in negative control deer obex (B lane 3). PrP^CWD detection is demonstrated in CWD positive control deer obex (B lane 2).