Table 1. Constituents of the optimal growth medium.

Medium Components	Final Concentration
Hepes Buffer (Lonza)	10 mM
Pen/Strep (Lonza)	100 U/mL
B27 without Vit A (Gibco)	2 mL (50X)/100 mL Culture Medium
bFGF (R&D Systems)	10 ng/mL
TGF-α (R&D Systems)	20 ng/mL
Heparin (Leo Pharma AS)	2.5 µg/mL
FBS (PAA Laboratories)	1%
DMEM/F12 (Life Technologies, Inc)	1X