Table 2. A proteomic comparison was made between SVZ and HPC cultures.
Protein | Gene Symbol | Protein | mRNA |
Up in SVZ | HPC/SVZ | HPC/SVZ | |
Ras-related protein R-Ras2 | RRAS2 | 0.40387 | 0.76 |
Collagen alpha-1(I) chain | COL1A1 | 0.40303 | 0.94 |
Filamin-B | FLNB | 0.53199 | 1.01 |
Microtubule-associated protein 1B | MAP1B | 0.53036 | 1.09 |
Neurofilament medium polypeptide | NEFM | 0.25961 | 0.93 |
Collagen alpha-1(XVIII) chain | COL18A1 | 0.27087 | 1.04 |
Filamin-C | FLNC | 0.55772 | 0.90 |
LIM domain only protein 7 | LMO7 | 0.57999 | 1.00 |
Heat shock-related 70 kDa protein 2 | HSPA2 | 0.32062 | 1.06 |
Collagen alpha-2(I) chain | COL1A2 | 0.60116 | 1.00 |
Glycogen phosphorylase, brain form | PYGB | 0.63077 | 0.89 |
Eukaryotic translation initiation factor 5B | EIF5B | 0.69052 | 1.03 |
Up in HCP | |||
Mas-related G-protein coupled receptor member F | MRGPRF | 2.0517 | 0.83 |
Phosphate carrier protein, mitochondrial | MCART1 | 1.7571 | 1.04 |
NAD(P)H dehydrogenase [quinone] 1 | NQO1 | 1.7365 | 1.13 |
Histone H1.2 | HIST1H1C | 1.7194 | 0.96 |
Fatty acid desaturase 2 | FADS2 | 1.7104 | 0.90 |
Glucose-6-phosphate 1-dehydrogenase | G6PD | 1.6318 | 1.10 |
Histone H1.5 | HIST1H1B | 1.4966 | 0.99 |
Argininosuccinate synthase | ASS1 | 1.4611 | 1.26 |
Collagen alpha-2(VI) chain | COL6A2 | 1.4224 | 1.24 |
Splicing factor, arginine/serine-rich 7 | SFRS7 | 1.3969 | 0.96 |
A proteomic comparison was made between SVZ and HPC cultures for differentially expressed proteins using ‘Silac’ technology that discriminates proteins synthesized in culture incorporating ‘heavy’ amino acids. Data were obtained for 840 proteins. Twelve of these were shown to be upregulated >1.4 fold in SVZ and 11 were shown to be upregulated >1.4 fold in HPC (p<<0.05, n = 3). Column 4 indicates whether this upregulation is reflected in the RNA microarray data (bolded ratios are those that are not).