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. 2013 Aug 14;8(8):e71480. doi: 10.1371/journal.pone.0071480

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© 2013 Saito et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) 3′-DIG–labeled LNA probe was utilized to detect the localization of miR-196a in laryngeal cancer tissues by in situ hybridization. Negative control without miR-196a-specific probe is shown (right panel). Boxed fields are presented with higher magnification in panel B. (B) miR-196a was detected both in cancer cells (right panel; filled arrowheads) and stroma (right panel; grey arrowheads), while not detected in normal squamous cell epithelium (left panel; white arrowheads). The scale bar (black) represents 200 µm.