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. 2013 Aug 14;8(8):e72592. doi: 10.1371/journal.pone.0072592

Figure 8. Silencing of LvIAP1 and LvIAP3 facilitates the reproduction of WSSV.

Figure 8

At 48 hours after dsLvIAP1, dsLvIAP3, dsGFP or PBS injection, the shrimp were infected intramuscularly with a WSSV inoculum (107 copies/shrimp). At the indicated time points after WSSV infection, the gills of these shrimp were collected for qPCR analysis. The mRNA expression level of WSSV VP28 in the gills of shrimp injected with PBS, dsGFP (control), dsLvIAP1, or dsLvIAP3 after WSSV infection were determined using qPCR. The mRNA expression level of WSSV VP28 was normalized to that of LvEF-1α using the relative standard curve method for calculation of changes in gene expression as described in previous studies [43]..