Figure 10. Activation of the promoters of Drosophila and shrimp AMP genes by overexpression of LvIAP2 in Drosophila S2 cells.

Drosophila S2 cells were transfected with a protein expression vector (pAC5.1 empty vector or pAC5.1-LvIAP2 vector), a luciferase reporter plasmid (pGL3-Basic, pGL3-PEN453, pGL3-PEN309, pGL3-PEN4, pGL3-Drs, or pGL3-AttA), and pRL-TK Renilla luciferase plasmid (as an internal control) (Promega, USA). Thirty-six hours later, the cells were harvested for examination of luciferase activities using the dual luciferase reporter assay system (Promega, USA). All data are representative of three independent experiments. The bars indicate the mean ± S.D. of the luciferase activity (n = 3).