Table 1. PCR primers used in this study.
| Primer | Primer sequence (5’–3’) | |
|---|---|---|
| cDNA cloning | ||
| LvIAP1-5’ RACE1 | CATCCTATTGCCAGTTCATCC | |
| LvIAP1-5’RACE2 | TGACCTTGTCCGTTGCTTTG | |
| LvIAP1-3’ RACE1 | GAAGATGGCTGCTGCTGG | |
| LvIAP1-3’RACE2 | TGACTTGGAAATGTACCGACAG | |
| LvIAP2-5’ RACE1 | CGTCATCTCCTTTCTTCGTGTA | |
| LvIAP2-5’ RACE2 | TCTCGTACCTCAGGCTATCGTA | |
| LvIAP2-3’ RACE1 | ATGCCGAGATGGATGTTGTG | |
| LvIAP2-3’ RACE2 | CTCAGTGCCCCATCTGTAGGA | |
| LvIAP3-5’ RACE1 | TCATTCCTAAAGTCAATCGTGCT | |
| LvIAP3-5’ RACE2 | CTAAGTCATCAGGGGATAACCAAT | |
| LvIAP3-3’ RACE1 | TGTTAATGAGCCAGATAGCACG | |
| LvIAP3-3’ RACE2 | GCCACGTTACATTTTGTAGGTCAG | |
| qPCR analysis | ||
| qPCRLvIAP1-F | GAGATGAGCACAGAGGAAAAGAG | |
| qPCRLvIAP1-R | ATGGATGAACTGGCAATAGGA | |
| qPCRLvIAP2-F | CCCGCACTGTCCATTTATCA | |
| qPCRLvIAP2-R | GCCTTGACGTTCCACATTCA | |
| qPCRLvIAP3-F | GGAACATACCTTTGGTTAGGAGTC | |
| qPCRLvIAP3-R | TCAATCGTGCTATCTGGCTCA | |
| qPCRLvEF-1α-F | GAAGTAGCCGCCCTGGTTG | |
| qPCRLvEF-1α-R | CGGTTAGCCTTGGGGTTGAG | |
| dsRNA preparation* | ||
| dsGFP-F | AGTGCTTCAGCCGCTACCC | |
| dsGFP-R | GCGCTTCTCGTTGGGGTC | |
| dsGFP(T7)-F | T A A T A C G A C T C A C T A T A G GAGTGCTTCAGCCGCTACCC | |
| dsGFP(T7)-R | T A A T A C G A C T C A C T A T A G GGCGCTTCTCGTTGGGGTC | |
| DsLvIAP1-F | AGCAAGGGTTTGGAGAACTTCT | |
| dsLvIAP1-R | ATGACAAAGGATAAAGAAAGAGAGG | |
| dsLvIAP1(T7)-F | T A A T A C G A C T C A C T A T A G GAGCAAGGGTTTGGAGAACTTCT | |
| dsLvIAP1(T7)-R | T A A T A C G A C T C A C T A T A G GATGACAAAGGATAAAGAAAGAGAGG | |
| dsLvIAP2-F | CGCTTGGTAGACAGGCTAAGAT | |
| dsLvIAP2-R | TACACGAAGAAAGGAGATGACG | |
| dsLvIAP2(T7)-F | T A A T A C G A C T C A C T A T A G GCGCTTGGTAGACAGGCTAAGAT | |
| dsLvIAP2(T7)-R | T A A T A C G A C T C A C T A T A G GTACACGAAGAAAGGAGATGACG | |
| dsLvIAP3-F | ATCACCTGTCTCCCATTTACCT | |
| dsLvIAP3-R | TCATGAAGTGGGAGAAGGGTAA | |
| dsLvIAP3(T7)-F | T A A T A C G A C T C A C T A T A G GATCACCTGTCTCCCATTTACCT | |
| dsLvIAP3(T7)-R | T A A T A C G A C T C A C T A T A G GTCATGAAGTGGGAGAAGGGTAA | |
| protein expression** | ||
| pA5.1LvIAP1-F | CGGGGTACCATGACAAAGGATAAAGAAAGAGAGG | |
| pA5.1LvIAP1-R | GCTCTAGAAGCAAGGGTTTGGAGAACTTCT | |
| pA5.1LvIAP2-F | AAGGAAAAAAGCGGCCGCCGCCACCATGGGTGATATGTC-CCACGATC | |
| pA5.1LvIAP2-F | GCTCTAGAGGAGACAATAGGTTTGATGGTGAAT | |
| pA5.1LvIAP3-F | CGGGGTACCATGGCTCTATTAGATGACCATATGG | |
| pA5.1LvIAP3-F | GCTCTAGACTTTGGAATATTACCAACTGGTTTTC | |
* T7 RNA polymerase binding site is underlined; ** primers used in the cellular localization and luciferase reporter assays were the same.