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. 2013 Aug 14;8(8):e72468. doi: 10.1371/journal.pone.0072468

Figure 5. Correlation between galectin-7 expression and NF-κB activity.

Figure 5

(A) Putative binding sites for NF-κB, c-Rel and p53 within the proximal promoter region of the human galectin-7 gene. The location of the putative binding sites were obtained by analyzing the sequence of the galectin-7 promoter using the TFSearch program which searches highly correlated sequence fragments against TFMATRIX transcription factor binding site profile database in the 'TRANSFAC' databases by GBF-Braunschweig (http://www.cbrc.jp/research/db/TFSEARCH.html). (B) Galectin-7 protein levels measured by ELISA in MDA-MB-231, MDA-MB-453, MCF-7 and MDA-MB-468 breast cancer cell lines. Human epithelial keratinocytes (HaCaT) was used as a positive control. Similar results were obtained by Western blot analysis (Figure S4). (D) NF-κB and p53 activity as measured following transient transfection with luciferase reporter vectors in breast cancer cell lines with different p53 status and HaCaT cells. Transfection efficiency was normalized with co-transfection with a β-galactosidase reporter vector.