Figure 5. Chemokine and cytokine mRNA expression of bovine monocyte subsets.

Bovine MACS-separated cM, intM and ncM (n = 3 animals) were stimulated with LPS (1 µg/ml) for 3 h. Messenger RNA copy numbers of the chemokines CXCL1 and CXCL8 and the cytokines IL-1β, Arginase1, iNOS and TNF-α were determined in 8 ng total RNA by quantitative RT-PCR. Samples were tested in duplicates. Differences between treatments and between groups (one-way ANOVA) were considered significant (*) at p<0.05.