Figure 2. Sorting preference of the abcB4⁻ and abcC13⁻ mutants in chimeric slugs.

We co-developed unlabeled mutant cells with GFP-labeled wild-type cells. Pictures were taken at 16-hours of development. In chimeric organisms, the wild-type cells (A) were evenly distributed throughout the slug; the abcB4⁻ (B) and abcC13⁻ (C) mutant cells preferentially sorted to the prestalk area and formed most of the pstO cells (arrowheads). Panels A and B are representative images whereas panel C represents fewer than half of the chimeric slugs observed.