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. 2013 Aug 14;8(8):e70832. doi: 10.1371/journal.pone.0070832

Figure 7. Immune-fluorescence tests for Ki67 (100×) and Propidium Iodide FACS analysis.

Figure 7

P0 and P3 were harvested following 8 days of culture. Ki67 was used as a marker for cell proliferation. In a process known as cycle analysis, propidium Iodide FACS analysis was used to measure the DNA content of cells and distinguish between cells in G1, S, and G2/M. The results showed that P0 and P3 cells were positive for Ki67 and there were a large number of cells in S and G2/M, confirming the existence of proliferation phenomena in P0 and P3. (Blue: DAPI, Green: FITC beads).