TABLE 1.
Identified genes coding for the enzymes of the variant of the EMP pathway in T. tenax
| Enzymea | EC no. | COG no.b | Gene | Accession no. | Evidence value and best hitc | Reference(s) |
|---|---|---|---|---|---|---|
| Hexokinase (HK) | 2.7.1.1 | 1940 | hxk | AJ510140 | 3e-99 P. aerophilum, glucokinase, (ROK family) | 11 |
| Glucose-6-phosphate isomerase (PGI) | 5.3.1.9 | 0166 | pgi | AJ621272 | le-93 P. aerophilum | |
| PPi-dependent phosphofructokinase (PPi-PFK) | 2.7.1.90 | 0205 | pfp | Y14655 | 0.0 T. tenax | 56 |
| Fructose-1,6-bisphosphatase type IV (FBP)/inositol-1-monophosphatase (IMP) | 3.1.3.11/ 3.1.3.25 | 0483 | fbpA/suhB | le-63 P. aerophilum, extragenic suppressor protein SuhB homolog | ||
| Fructose-bisphosphate aldolase (FBPA) | 4.1.2.13 | 1830 | fba | AJ310483 | 1.0e-148 T. tenax | 54 |
| Triosephosphate isomerase (TIM) | 5.3.1.1 | 0149 | tpi | AJ012066, AJ515539 | 1.0e-120 T. tenax | 74 |
| Glyceraldehyde-3-phosphate dehydrogenase (nonphosphorylating) (GAPN) | 1.2.1.9 | 1012 | gapN | Y10625 | 0.0 T. tenax | 4, 5, 21, 40 |
| Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (GAPDH) | 1.2.1.13 | 0057 | gap | Y10126 | 1.0e-135 P. aerophilum | 5, 21 |
| Glyceraldehyde-3-phosphate oxidoreductase (ferrodoxin dependent) (GAPOR) | 2414 | gor | AJ621330 | 0.0 P. aerophilum, aldehyde ferredoxin oxidoreductase | ||
| 3-Phosphoglycerate kinase (PGK) | 2.7.2.3 | 0126 | pgk | Y10126 | 1.0e-124 P. aerophilum | 5 |
| Phosphoglycerate mutase (PGAM) | 5.4.2.1 | 3635 | gpmA | AJ6212333 | 1e-80 P. aerophilum, phosphonopyruvate decarboxylase | |
| Enolase (ENO) | 4.2.1.11 | 0148 | eno | AJ621325 | 1e-50 P. aerophilum | |
| Pyruvate kinase (PK) | 2.7.1.40 | 0469 | pyk | AF065890 | 0.0 T. tenax | 48 |
| Phosphoenolpyruvate synthetase (PEPS) | 2.7.9.2 | 0574 | pps | AJ515537 | 0.0 P. aerophilum | 63, Tjaden et al., unpublished |
| Pyruvate, phosphate dikinase (PPDK) | 2.7.9.1 | 0574 | ppdk | AJ515538 | 0.0 P. aerophilum | 63, Tjaden et al., unpublished |
a
Enzymes exclusively catalyzing the catabolic pathway are underlined; those operating exclusively in the anabolic direction are shown in bold. For enzymes that are biochemically characterized, the respective references are given. The abbreviations in parenthesis correspond to the respective enzymes shown in Fig. 1.
b
COG, Clusters of Orthologous Groups (http://www.ncbi.nlm.nih.gov/COG).
c
Evidence values represent the best Blastp hits and respective species; for homologs with functions deviating from those of the assigned T. tenax enzymes, the respective annotations are given.