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. 2013 Aug 14;8(8):e72464. doi: 10.1371/journal.pone.0072464

Figure 5. EF24 regulates radiation-induced telomerase activation in human neuroblastoma cells.

Figure 5

Representative gels and corresponding densitometry analysis showing telomerase activity in (A) MC-IXC, (B) SH-SY5Y, (C) SK–PN–DW and (D) IMR-32 cells either mock-irradiated; exposed to 2Gy and harvested after 6, 24, 48 and 72h; treated with 10, 50, 100 and 200nM EF24 for 3h followed by IR exposure and harvested after 3h, treated with 200nM EF24 for 34 followed by IR exposure and harvested after 1, 3, 6, 12, 24, 48, 72 and 96h; transfected with p50/p65 for 24h or transfected with p50/p65 for 24h and treated with EF24 for additional 24h. Densitometry analysis with automatic band detection (ImageQuant TL, Amersham Biosciences) showed significant inhibition of either 2Gy- or p50/p65-induced telomerase activity with EF24.