Figure 5. Structure-activity-relationship study of pyrazolopyrimidine inhibitors of APH(3’)-Ia.
A) Chemical structures of parent compounds and derivatives grouped by modification site. B) Measured inhibitory constants (Ki) of compounds against APH(3’)-Ia. C) Chemical synthesis scheme for compounds NT6-66 and NT6-67. Method adapted from Todorovic, N., Awuah, E., Shakya, T. & Wright, G. Microwave-assisted synthesis of N1-and C3-substituted pyrazolo [3, 4-d] pyrimidine libraries. Tetrahedron Letters (2011).