FIG. 6.

SrtB-mediated anchoring of SvpA is not required for bacterial growth in macrophages. RAW 264.7 macrophages were exposed to wild-type EGD-e bacteria (squares) or to the ΔsrtA (circles), ΔsrtB (triangles), or svpA (diamonds) mutant for 15 min at 4°C. After 45 min of internalization at 37°C, gentamicin was added to kill extracellular bacteria, and bacterial survival was monitored for 8 h postinfection. In contrast to that of the svpA mutant, the intracellular multiplication of the ΔsrtB mutant was not affected.