FIG. 4.
SDS-polyacrylamide gel of cell extracts from D. multivorans grown on [75Se]selenite-containing mineral medium. (A and B) Autoradiographs of an SDS-10% polyacrylamide gel (A) and an SDS-12.5% polyacrylamide gel (B). (C) 100-kDa section of the Coomassie blue-stained SDS-12.5% polyacrylamide gel. Lanes 1 to 4 and 7, crude extract of cells grown on benzoate (lane 2, as lane 1, but after treatment of cell extract with proteinase K; lane 3, crude extract after treatment with RNase A); lanes 5 and 8, crude extract of cells grown on lactate; lane 6, 97-kDa molecular mass standard. The protein migration front in panel B is marked by an arrow. The arrow in panel C points to an ∼100-kDa protein band which is differentially expressed in cells grown on benzoate and lactate.