FIG. 5.

Two-dimensional SDS-PAGE of proteins from cell extracts of D. multivorans. (A) Coomassie blue-stained two-dimensional SDS-polyacrylamide gel of extracts from cells grown on benzoate. The rectangles mark the positions of the radioactively labeled protein bands. (B) Autoradiograph of a two-dimensional SDS-polyacrylamide gel of extracts from cells grown on benzoate and [⁷⁵Se]selenite. The vertical dimension is an SDS-10% polyacrylamide gel; the horizontal dimension is isoelectric focusing using a pH gradient as indicated.