TABLE 1.
Purification of benzoate CoA ligase from D. multivoransa
| Purification step | Amt of protein (mg) | Total activity (U) | Sp act (U mg−1) | Enrich- ment (n-fold) | Recovery (%) |
|---|---|---|---|---|---|
| 100,000 × g supernatant | 470 | ND | |||
| (NH4)2SO4 precipitation | 125 | ND | |||
| DEAE-Sepharose | 6.3 | 7.8 | 1.2 | 1 | 94 |
| Q-Sepharose | 4.5 | 8.3 | 1.8 | 1.5 | 100 |
| Reactive Green | 2.4 | 6.9 | 2.9 | 2.4 | 82 |
| Mono Q | 0.3 | 2.4 | 8.2 | 6.8 | 34 |
a
The enzyme was purified from 10 g (wet mass) of cells of D. multivorans grown on benzoate under sulfate-reducing conditions. One unit corresponds to 1 μmol of benzoyl-CoA formed per min. Due to high background reaction, benzoate CoA ligase could not be measured after the first two purification steps (indicated as ND [not determined]).