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Real-time RT-PCR analysis of intron splicing from pCOM9 and intron mutant derivatives. The relative fluorescence at the end of each PCR cycle was plotted for each sample, and a threshold of 0.8 U of fluorescence was selected (dashed line) to calculate the CT values. The amplification plot is the plot for one RT-PCR trial and is representative of the results for the three trials used to calculate the relative amount of spliced mRNA.
