TABLE 2.
Comparison of plasmid transfer frequencies and mRNA splicing for pCOM9 and intron mutants
| Plasmida | Relative plasmid transfer frequencyb | Relative amt of spliced mRNAc |
|---|---|---|
| pCOM9 | 100 (12) | 100 |
| pCOM-G471A | 1.83 ± 0.49 (4) | 2.73 ± 1.80 |
| pCOM-G2399A | 1.27 ± 0.79 (4) | 4.22 ± 0.75 |
| pCOM-A2486C | 6.15 ± 5.44 (3) | 1.43 ± 1.44 |
| pCOM-A2486Δ | 0.45 ± 0.27 (4) | 0.35 ± 0.15 |
a
L. lactis MMS370(pM1014) carried the plasmids indicated.
b
Frequency of pM1014 plasmid transfer from donor to recipient as determined by quantitative mating assays. The plasmid transfer frequency achieved by complementation with pCOM9 was defined as 100, and the transfer frequency for each of the mutants is expressed relative to the frequency observed for pCOM9. The values are means ± standard deviations for the actual numbers of replicate experiments indicated in parentheses.
c
Relative amount of spliced mRNA as determined by real-time RT-PCR. The values are means ± standard deviations for three replicate experiments.