TABLE 3.

Plasmid transfer frequencies and mRNA splicing of A30-6 and subclones

Intron mutation(s)	Relative plasmid transfer frequencya	Relative amt of spliced mRNAb
A2396G, U2428Cc	0.06 ± 0.02	1.4 ± 1.3
A2396Gd	72.4 ± 4.2	130.0 ± 31.7
U2428Cd	32.8 ± 9.9	174.9 ± 39.9
A2396G, U2428Cd	0.08 ± 0.04	0.22 ± 0.05

^aFrequency of pM1014 plasmid transfer from donor to recipient as determined by quantitative mating assays. The plasmid transfer frequency achieved by complementation with A30-6 is expressed relative to the level of parent vector pCOMT-Kan transfer. The transfer frequencies of the remaining plasmids are expressed relative to the pCOM9 transfer frequency. The values are means ± standard deviations for two replicate experiments.

^bRelative amount of spliced mRNA as determined by real-time RT-PCR. The values are means ± standard deviations for four replicate experiments.

^cL. lactis MMS370 (pM1014) carried the pCOM-A30-6 vector.

^dL. lactis MMS370 (pM1014) carried a pCOM9-based vector with the mutation(s) indicated in the intron.