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. Author manuscript; available in PMC: 2014 Apr 5.
Published in final edited form as: J Proteome Res. 2013 Mar 15;12(4):1789–1796. doi: 10.1021/pr3011342

Figure 3. The 47-kDa protein is a cytoplasmic or perinuclear protein, as revealed by immunofluorescence analysis of HEp-2 cell substrate using human liver fibrosis sera.

Figure 3

Immunofluorescence was done using patients’ sera or sera purified with 47-kDa antigen (see Materials and Methods). Left panel (A, C, E, G): pre-absorption sera; right panel (B, D, F, H): post-absorption sera. Three representative liver fibrosis sera (D, F and H) were used, and one normal human serum (A) was used as control.